There are dedicated kits for Sepsis; The MALDI Biotyper Sepsityper®, which enable a proteomic profile to be established, with high resolution mass spectrometry that applies sequence libraries for a positive identification of various bacteria directly from positive blood cultures.
The concentrations of malondialdehyde (MDA), sodium oxide dismutase (SOD) and nitric oxide (NO) were assessed using dedicated kits (Nanjing Jiancheng Biological Engineering Institute, China) according to the manufacturer's protocols.
DNA was extracted from ethylenediaminetetraacetic acid-treated peripheral blood using an automated methodology (Maxwell 16; Promega, Madison, WI, USA) and dedicated kits (Maxwell 16 Blood DNA Purification Kit; Promega).
"A lot of people abandon their activity trackers after a few months because it's something they have to take off and charge and it's not familiar to them - they tend to lose them or forget to put them back on," says Ariel Carpenter, marketing manager at the French firm, which also sells such dedicated kit.
Nevertheless we noticed that these levels were often higher than the levels of methylation of the same FFPE DNA extracted with the dedicated kit because 40.0% of the cases showed a significantly higher level of methylation (Additional file 2).
IgG levels were determined in the plasma by quantitative ELISA sandwich using a pig dedicated ELISA kit (Bethyl Pig IgG ELISA quantitation kit, Interchim, Montluçon, France).
Haptoglobin concentrations were determined in the plasma by a pig dedicated colorimetric kit (Tridelta Ltd ,Maynooth, Ireland).
Blood is collected in PaxGene DNA tubes (Qiagen) and the DNA is purified using a dedicated purification kit.
HMGB1 assays were performed on a dedicated ELISA kit and performed on mouse serum to manufacturer specifications (IBL International GMBH, Hamburg, Germany).
That RNA is co-extracted using the Qiagen kit may seem surprising (given the kit is marketed by the vendor as a DNA extraction kit, and that considerably more expensive commercially available dedicated RNA extraction kits also exist).
To avoid metal contamination, blood samples were drawn using a sampling kit dedicated to trace element determination; this kit included an S-Monovette needle (ref. 85.1162.400; Sarstedt, Marnay, France) and lithium heparin in a 7.5-mL S-Monovette for trace metal analysis (ref. 01.1604.400, Sarstedt).
