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Increasing concentrations of trypsin and NCPCs-DOX correlated with decreasing of cell viability from 69 to 56%% in case of incubation with the UDD-DOX (Fig. 9, groups 1 4, UDD-DOX, HT29) and from 91 to 75%% in case of incubation with OLC-DOX (Fig. 9, groups 1 4, OLC-DOX, HT29).
It is known that damage to cell membranes causes the decreasing of cell viability through peroxidation of membrane lipids.
Therefore, PMS reducing the migration and invasion ability of cancer cells is not due to the decreasing of cell viability.
In addition dying cells (green but fading of colour) and decreasing of cell density were also observed.
As shown in Figure 6(a), CNE2 was sensitive to cisplatin with decreasing of cell viability in a dose-dependent manner and the IC50 (inhibitory concentration 50) was about 3 μmol/L.
Inducing the osteogenic differentiation caused as expected, the decreasing of cell number after 4 and 12 days of cultivation by comparison with DF stem cells cultivated with stem cells medium but with no statistical differences.
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Thus, the dedifferentiation of chondroid cells was relative to the decrease of cell surface proteins.
The increase or decrease of cell length results in the area of electrochemical reaction.
However, a decrease of cell viability was observed in cultures treated with ASNase.
25 50 μM B10 resulted in a significant decrease of cell viability and BrdU incorporation.
Only in the case of their combined action dose dependent decrease of cell viability was detected at 24 h.
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