Sentence examples for decreasing km from inspiring English sources

On the other hand, when activities of the recombinant uricase were decreasing, Km by measuring absorbance at 308 nm in borate buffers decreased to stable values (Figure 2).

Here, we use Walker A and B mutants to demonstrate that D1 is capable of hydrolyzing ATP and show for the first time that nucleotide binding in the D2 domain increases the catalytic efficiency (kcat/Km) of D1 ATP hydrolysis 280-fold, by increasing kcat 7-fold and decreasing Km about 40-fold.

This would enhance the binding of pABA to the active site, thereby decreasing Km, but would also slow the release of product, thereby decreasing Vmax.

Nano-TiO2 addition decreased Km and increased Vmax of CLEAs.

These observations may explain the significantly decreased KM and increased kcat/KM values of this variant toward all substrates recently tested in experiment.

The higher catalytic efficiency of D77N toward C4 substrate was due to a combination of decreased Km and considerably increased kcat.

As shown by the results, the mutant K312E had a decreased Km and increased kcat, which resulted in a ~ 2.5-fold improvement in the catalytic efficiency.

In contrast, F295A also spares the Km for ATCh, but decreases Km for PTCh and BTCh 4-fold and 33-fold, respectively.

In H. sapiens AChE, F295L has little effect on the Km for ATCh or PTCh but decreases Km for BTCh 10-fold [12].

We have shown that compound 4 decreased KM, kcat (compared with no inhibitor) and decreased the kcat/KM implying uncompetitive inhibition.

However, the majority of the TH mutants had decreased Km values for BH4 and increased Km values for tyrosine compared with wt TH.