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The presence of H. pylori in stomach tissues was identified by visualization of spiral microorganisms in histology sections and/or by immunohistochemistry. Four-micron tissue sections were de-waxed in xylene and decreasing grades of ethanol.
For the tissue sections, we followed the same procedure as mentioned previously after removing paraffin using xylene and hydrating the section using decreasing grades of alcohol (100%to20%0%).
The alcohol-fixed smears were hydrated through decreasing grades of alcohol, washed in phosphate buffered saline (PBS) for 10 min, and treated with 3%H2O22 for 15 minutes and then incubated with in-house absorbed polyclonal anti-MPT64 antibody (received from the University of Bergen, Norway) at 1/250 dilution.
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A few horses had an unexpected decreased grade of OUC at second blinded examination.
Four-micron sections of formalin-fixed, paraffin-embedded (FFPE) tissues were deparaffinised in xylene for 5 minutes and gradually rehydrated in decreasing grades (100%, 90 %, 80 % and 70 of industrial methylated spirits (IMS) and water washes.
First, the surfaces were mechanically polished with abrasive papers (grades 500, 1200, 2400, and 4000) followed by diamond pastes of decreasing grades (3, 1, and 0.25 m).
Grade 3 4 convulsion and decreased level of consciousness was seen in only four patients who were all from arm I.
Finally, grade 3 4 convulsions and decreased level of consciousness were only seen in four patients, all of whom were from arm I.
Decreasing the grades of evidence based on these three criteria is thus justified although there is a need to consider the weighting of these criteria.
Formalin-fixed paraffin embedded tissues were deparaffinized in xylene and rehydrated in a decreasing graded series of ethanol.
If the risk of bias in all randomized trials is very high indeed, decreasing the grade of evidence by only two grades (e.g. evidence quality changes from high level to low level) may not be enough, but rather the most appropriate decision could be to decrease the grade of evidence by three grades (e.g. evidence quality move from high level to very low level).
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