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Before staining, 5-µm-thick tissue sections were dewaxed in xylene, rehydrated through decreasing concentrations of ethanol, washed in PBS and stained with H&E.
This is in contrast to the risk of reversion which increases with decreasing concentrations of formaldehyde.
For warming, oocytes were exposed to decreasing concentrations of trehalose 0.3, 0.2 and 0.1 M for IVM.
Decreasing concentrations of biotinylated ELR+ CXC chemokines up to five- and ten-fold below the measured KD were used for each round of selection in order to select for crossreactive clones with improved affinity.
We then promoted the selection of clones displaying increased binding affinity by allowing the mutants to evolve through sequential cycles of equilibrium-based selection using decreasing concentrations of ELR+ CXC chemokines.
The MIT team speculate that the rise in methane may be a function of decreasing concentrations of hydroxyl, the scavenger radical that mops up methane, perhaps as well as increasing emissions.
Instead, the presence of ammonium ion spectra demonstrates decreasing concentrations of these SAS.
A series of decreasing concentrations of drug-fortified solutions was analysed to determine LOD and LOQ.
Decreasing concentrations of each antibiotic and their corresponding Gnps conjugated form were added to the respective tubes.
Sections were deparaffinized with xylene, rehydrated with decreasing concentrations of ethyl alcohol and finally PBS.
The chosen starvation conditions were (i) HOMEM with decreasing concentrations of FCS and (ii) PBS only.
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