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To test this, a decreasing amount of the plasmid DNA encoding for wild-type V5-MKK7 was transfected into CHO K1 cells and the cleavability of MKK7 was tested in cells in the presence of cycloheximide.
A decreasing amount of the mineral was used as the environmental stimulus of the network model, reflected by the perturbation function u(t) (see Methods).
A similar behavior, decreasing amount of the RS phase with exchange time, is visible for the medium (cyan symbols) and small (green symbols) sized core/shell samples, but here already the first formed shells depict a significantly lower amount of CdS in the RS phase.
With an increasing amount of vermiculite and a decreasing amount of the catalyst (TiO2), the absorbability is enhanced, whereas the catalytic ability is reduced at the same time.
With increasing T a, on the one hand, σ sg decreased as a result of structural relaxation in the amorphous substrate, and on the other, εsl might be weakened and thus σ sl increased because of local thermally activated bonding between the atoms such as Fe-B and Fe-Si in the matrix, as witnessed by the decreasing amount of the FeSn2 phase formed at the interface (e.g., Figure 4d).
This multivariate statistical method creates a series of orthogonal axes to identify trends that explain the data variation, with each subsequent axis explaining a decreasing amount of the variation.
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This effect was gradually diminished when decreasing amounts of the PTB 1 plasmid were used in the transfection assay.
The results shown in Fig. 2 demonstrate that amplification of decreasing amounts of the competitor with fixed amounts of total RNA resulted in signals of decreasing intensity.
Hence, the decreased amount of the three mitochondrial components may indicate reduced production of free radicals, although the functionality of the mitochondria is yet to be confirmed.
On the contrary, overexpression of ADAM10 and its mutant even leads to a decreased amount of the inflammation marker calprotectin (the dimer of S100a8 and S100a9).
In hormone-dependent neoplastic cell lines, application of GnRH agonists resulted in desensitization due to the decreased amount of the membrane receptor and its internalization [ 21].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com