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ALDH1A3 KD modestly decreased sphere formation (25% reduction).
Treatment with AMPK inhibitor Compound C or knockdown of AMPK α2 dramatically decreased sphere formation in MCF7, MDAMB231 and BT 474 breast cancer cell lines.
Furthermore, overexpression of the nonphosphorylatable S116A mutant of PEA15 also dramatically decreased sphere formation in all the three cell lines compared to WT PEA15.
Decreased sphere formation, although to a lesser extent, was also detected in luminal MCF-7 cells following ATG4 A inhibition.
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Further, decreased sphere-formation in the doxorubicin-cultured non-SP cells may reflect the doxorubicin-mediated decrease in total cells expressing CD133.
Again, variability increased with decreasing sphere size and decreasing activity.
Variability in measurements increased with decreasing sphere size and decreasing activity.
For both FCC and SC packing structures, the areal capacitance (in μF/cm2) increased with decreasing sphere diameter.
Decreasing sphere volume required higher threshold levels to achieve accurate volume estimation.
The debonding stresses increased with decreasing sphere diameter, whereas the length to diameter ratio of the resulting matrix cracks increased with increasing sphere diameter.
For both PET VOI and exact VOI the COV of the recovery coefficient increases with decreasing sphere volume and after applying PVC.
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CEO of Professional Science Editing for Scientists @ prosciediting.com