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However, if reciprocal monophyletic species are mandatory this is bound to decrease identification success; see e.g. Fazekas et al. [7].
No threshold was defined since this may decrease identification of multiphosphorylated peptides.
We therefore strongly recommend that the ChIP data be compared with the corresponding input data to decrease identification of false-positive protein-binding sites.
Cufflinks was more likely to mistakenly identify intronic regions containing noise reads as genes, whereas Tiling Assembly's more conservative approach to exon identification decreased the identification of these false genes.
We addressed this problem by also analyzing an aliquot of unpurified plasma membrane samples with tandem mass spectormetry; however, the increased sample complexity of nonpurified samples can result in decreased peptide identification and inconclusive results, especially for less abundant proteins.
Conversely, for the microarray analysis, increasing the |fold change| cut-off from >1.5 to >2.0 dramatically decreased the identification of DEGs from 2,039 to 472 while increasing the P1 t) cut-off had a less dramatic effect (2,039 DEGs at |fold change| ≥ 1.5, P1 t) ≥0.8 compared to 1,434 DEGs at |fold change| ≥ 1.5, P1 t) ≥0.9999).
However, this accuracy is lower than when the identification is from among five randomly chosen items (which is.738), indicating that greater similarity among the alternatives decreases the identification accuracy.
The findings of this study suggest that the new packs are decreasing smokers' identification with their packs and making them think more closely about the health warnings contained on them, potentially moving them closer to cessation.
In the generic-level databases the percent identification decreased: For Encephalartos, 26 of 44 (59.1%) species identified uniquely; for Cycas, 11 of 12 (91.7%) species identified uniquely; and for Macrozamia, 8 of 8 (100%) species identified uniquely.
As expected, the likelihood ratio of this strain's identification decreased with its relative abundance.
When comparing moments 2 and 3, the proportion of correct item identification decreased significantly in two items: SRO hydration (P = 0.002, n = 22) and Send to hospital (P = 0.023, n = 22).
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