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Phase of Tc drop was delimited as followed: the onset of Tc decrease was determined as the first time point after which at least 3 successive bins of Tc decrease occurred; the end of Tc increase phase was determined as the time point occurring before the 3 successive bins of stable Tc.
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The relative quantity was determined as following.
The fold increase or decrease was determined relative to controls after normalizing to β-actin as housekeeping gene.
X-values were determined as above.
The thresholds were determined as above.
were determined as well.
The decrease in H2O2 was determined as the decrease in absorbance at 240 nm with a spectrophotometer (Metertec SP8001).
The increase or decrease in luciferase activity was determined as a function of the endogenous activity of each construct.
For APX activity, the decrease in ascorbic acid (AsA) concentration was determined as the decline in absorbance at 290 nm and activity was calculated by the extinction coefficient (2.8 mM−1 cm−1 at 290 nm) for AsA (Chao et al. 2012; Nakano and Asada 1981).
aExtracranial contamination was determined as a decrease in cerebral oxygen saturation during inflation of a pneumatic head cuff compared to baseline values [ 14].
For dichotomous analysis response was determined as a decrease in DAS28 of ⩾1.2 at 24 weeks 17 and also as according to the European League Against Rheumatism (EULAR) response criteria.
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