As iDC mature there is a decrease in their uptake of pathogens and decreased expression of pathogen recognition genes [42], [43] these would explain the reduced DC-SIGN expression and is supported by the presentation of maturation markers at 12 h (Fig. 2).
Our third hypothesis, that the modifiers might improve the poor penetration of doxorubicin and mitoxantrone through tumour tissue was based on previous studies indicating that drug penetration was largely through the extracellular space and that mechanisms that decreased cellular uptake of drugs improved their tissue penetration (Tunggal et al, 2000; Cowan and Tannock, 2001).
Hence, down-regulation of the small GTPases might potentially be sufficient to decrease uptake of a range of agents, and restoring RhoA and related factors could possibly reverse this, even if there is no obvious impact on expression of transporters.
However, pretreatment of cells with Filipin remarkably decreased the uptake of unconjugated cMLV particles (P < 0.01), whereas no inhibitory effect on their uptake was observed in CPZ-pretreated cells.
As shown in Figure 3 e), CPZ (10 μg/mL) significantly decreased the uptake of iRGD-cMLV particles in HeLa cells, while no significant inhibitory effect on their uptake was observed when cells were pretreated with Filipin (10 μg/mL).
Knock-down of CHC also significantly decreased the uptake of biotinylated cell surface PrPC (Fig. 1d, e, lower panel).
The decreased cardiac uptake of 123I meta-iodobenzylguanidine (MIBG) on myocardial scintigraphy suggested that the degeneration of the cardiac sympathetic nerve began in the early stage of PD, even before neuronal loss in the dorsal vagal nucleus48.
Feeding the animals a Gpo1 inhibitor to block the glycerol-3-phosphate shuttle significantly decreased the uptake of glucose (Fig. 3f), consistent with this shuttle being an important sink for NADH that allows glycolysis to continue at a high rate.
Polysaccharides are nowadays recognized for decreasing the uptake of particulate carriers by the mononuclear phagocytic system (MPS).
A method for the radiohalogenation of proteins resulting in decreased thyroid uptake of radioiodine.
