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The samples were irradiated with a red laser light (Maestro CCM, λmax = 661 nm) to decrease the absorbance of DMA solution to ca. 0.2 0.3.
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Carbon monoxide decreased the absorbance of CO3O4 moiety, while H2 not only decreased the absorbance of CO3O4 moiety but also increased plasmon absorbance of Au.
Thus, all studied samples (extracts and aqueous βCD complex solutions) show antioxidant activity by means of decreasing the absorbance of the sample-DPPH mixture at 517 nm; this decreasing is more significant in the case of undiluted samples and remains important also in the case of diluted ones.
The decrease of the absorbance of the DPPH solution indicates an increase in DPPH radical scavenging activity.
The decrease in the absorbance of H2O2 was recorded at 240 nm for 1 min.
Compared with this, there is only a negligible decrease in the absorbance of SEC-LDH.
The decrease in the absorbance of the resulting solution was then measured spectrophotometrically at ν 517 nm.
The positive mutant was screened by measuring the decrease in the absorbance of NADPH at 340 nm and high performance liquid chromatography (HPLC).
The decrease in the absorbance of the solution was due to the destruction of the homo- and hetero-polyaromatic rings present in the dye molecules.
The progress of the reduction of 4-NP was monitored spectrophotometrically as a decrease in the absorbance of the substrate 4-NP at 400 nm.
Enzyme activity was assayed at 30 °C by monitoring the decrease in the absorbance of NADH at 340 nm on an ultraviolet (UV /visible spectrophotometer (BioTek Instruments, Inc. Vermont, USA).
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