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CCK8 assay showed that downregulating the expression of SOX4 caused a significant decrease of the proliferation rate in CNE2 cells).
In addition, the highly tumourigenic, poorly differentiated SW480 cell line and the well-differentiated CaCo2 showed a comparable decrease of the proliferation rate upon DHHC9 overexpression.
Administration of Trastuzumab resulted in a decrease of the proliferation index (P=0.004), GLUT1 (P=0.04) and HER2 (P=0.01) expression.
Results are reported in Figure 2B,C and confirm the significant decrease of the proliferation rate of glioma cells overexpressing MXI1.
We first observed a decrease of the proliferation rate of U937-E cells upon AMD3100 treatment, as compared with untreated cells, which is further enhanced by miR-146a.
Interestingly, proliferation of MSS cell lines SW480 and CaCo2, 48 h post-DHHC9 transfection, showed a slight but significant decrease of the proliferation rate.
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Similarly, decreases of the proliferation marker Ki-67 in tumour samples have been correlated with mTOR inhibitor activity (Neshat et al, 2001; Tabernero et al, 2008).
This was associated with a reduced absolute number of SMCs in the media and a decreased expression of the proliferation marker Ki67.
This response was confirmed by finding a decreased level of the proliferation marker, Ki67 in muscle.
Photoactivation of hypericin inhibited cell proliferation reflected by decreased expression of the proliferation marker Ki-67 and cell-cycle arrest in the G0/G1-phase.
The quiescent tumour spheroids (TSQ) showed decreased expression of the proliferation marker Ki-67 and increased expression of the quiescence marker p27kip1 compared to proliferating spheroids (TSP).
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