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The biodegradability of the poly-l-arginine, poly-l-lysine, chitosan, alginic acid sodium salt, and dextrane sulfate which are also widely used in polyelectrolyte microcapsules formation will induce the QD diffusion out of the polymeric membrane that should result in decrease of the fluorescent properties of the microparticles [3, 11, 39, 48 52].
Thus a decrease of the fluorescent signals (FL2) indicates loss of MMP.
Secretion of a vesicle was detected by a rapid reduction of the fluorescent signal from its own maximum value to the background level in less than 200 ms. Often this decrease of the fluorescent intensity of the vesicle was accompanied by a lateral spread of the fluorescent marker in the extracellular space, seen as a cloud of NPY-mRFP.
Prolonged exposure of the yeast to the estrogenic fractions that showed superinduction did, contrary to E2, not result in a decrease of the fluorescent response.
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A coupled assay was used to monitor the activity of the mutants: upon cleavage of l-KDO, pyruvate is generated which is immediately reduced to lactate by lactate dehydrogenase (LDH), and the reaction was followed by the decrease in the fluorescent signal of NADH (excitation of 340 nm and absorbance at 450 nm).
We demonstrated that genetic ablation of cathepsin B results in suppression of tumor infiltrating pro-inflammatory cells, notable attenuation of polyposis, and a decrease in the fluorescent signal emanating from the lesions.
The decrease in the fluorescent substrate and increase of fluorescent product were monitored using a microplate reader (FLUOstar Omega, BMG) with excitation at 334 nm and emission at 440 nm for substrate, and excitation at 440 nm and emission at 590 nm for product.
Fig. 4b shows the decrease in diffusion coefficient of the fluorescent alginate from 10 min to 90 min after the start of penetration into the mucus.
Additionally one can clearly observe a decrease of the number of red fluorescent cells and an increase of the number of green fluorescent cells for a decreased number of viable cells in the different samples.
As shown in Figure 5(B), lithium treatment of HeLa cells resulted in a dose-dependent decrease of the poly(ADP-ribose) fluorescent signal intensity.
After 30 min incubation the clarity of the earlier bands decreased due to diffusion of the fluorescent product (Fig. 1A).
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