DOI: http://dx.doi.org/10.7554/eLife.09008.017 Within the inv1 regulatory region, the absence of S2P resulted in decreased acetylation and increased occupancy of histones, which was associated with a marked decrease of PolII occupancy.
We attribute this change to the decrease of the occupancy related hindrance factor overcompensates the increase of the rate coefficient.
Because of Spt5 depletion, promoter-proximal association of RNAPII was reduced subsequently in fogsk8 mutant embryos, resulting in observed decrease of RNAPII occupancy at 24 hpf.
Of note, Dnmt2−/− primary bone marrow cells showed a decrease of codon occupancy in the ribosome A-site for all Dnmt2 target codons in the ribosome footprint assay.
In budding yeast, a moderate decrease in histone expression influences the DNA damage response, whereas substantial reduction of histones leads to a genome-wide decrease of nucleosome occupancy and global transcriptional noise (Celona et al., 2011).
PG-11144 could bind to, and alter, chromatin structure, thus decreasing the ability of LSD1 to target the nucleosome at specific chromatin sites, thereby leading to a decrease of LSD1 occupancy at the SFRP2 promoter.
At the same time point, we detected a strong decrease of Rpb2 occupancy at the TAF2 and BUD5 genes, which are located within 4 kb from either side of the DSB.
Indeed, Che-1-depleted cells exhibited a strong decrease of p53 occupancy at p21, Gadd45a, Plk2 and Rrbm2b promoters, with a parallel increase of this protein onto Puma, Noxa, Bax and TP53Inp1 genes.
Knockdown TUG1 detected a loss of PRC2 binding and a decrease of H3K27 trimethylation occupancy.
A marked decrease of Pol II occupancy was observed at the ftz promoter, gene body and distal downstream region.
