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We also found that 53BP1 foci were induced in a LET -and fluence-dependent manner and fewer foci were induced in G0 cells than G1 cells, however, the decrease of foci in 24 h after irradiation was highly relevant to the type of particles.
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Silencing of IKKε in human breast cancer cells resulted in a decrease of focus formation potential and clonogenicity as well as in vitro cell migration/invasion capabilities.
This resulted in a dramatic decrease of BRCA1/BARD1/RAD51 foci, but not of γH2AX foci (Fig. 9A, top and bottom panels).
We did not observe a general decrease of Rad52 foci in nmt41-xlf1.AA.
The only noticeable difference was a twofold decrease of RNaseY foci located at the septal region (Table 2).
Knockdown of SPOP also resulted in a decrease of RAD51 foci, suggesting a selective loss of function of SPOP-F133V in HDR.
It has been shown that the decrease of γH2AX foci number per cell after IR correlates with the rejoining of DSBs measured by pulse field gel electrophoresis (PFGE) (Löbrich et al. 2010).
In order to decrease the out-of-focus excitation, one needs to increase the DG groove density or the objective NA.
Reduction of blur via aberration correction decreases depth of focus and increases the symmetry in the through focus modulation transfer function [ 6, 24, 25].
Multiphoton microscopy has several advantages over confocal and traditional fluorescence microscopy, including decreased out of focus photodamage, increased imaging depths and intrinsic optical sectioning [11].
In contrast, this frequency persistently decreased the level of foci.
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