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Decrease in transformation frequency with increase in bacterial intensity was observed.
The decrease in transformation temperature causes a change in the morphology of intragranular ferrite from idiomorphic ferrite to acicular ferrite.
This identified a single NovSNalS recombinant clone (RR4108, from the unselected set, * in Figure 2C) with a reproducible 10-fold decrease in transformation (t-test p-value = 0.025).
The original work on the function of comF operon in DNA uptake revealed that a transposon insertion in comFB resulted in a 10-fold decrease in transformation efficiency and that was attributed to a polar effect on comFC expression [ 7].
In the prior work, ComFA and ComFC were demonstrated to be important for the DNA uptake process; deletion of ComFA resulted in a decrease in transformation efficiency by three orders of magnitude.
However, in our experiments, deletion of comFB comFC or comFC alone resulted in a 300-fold decrease in transformation efficiency that is about 30 times larger than the previously observed effect.
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With the other R. solanacearum strains as recipients (Figure 5), the transformation frequencies were systematically lower than with GMI1000 whatever the DNA positions tested and higher genomic divergence between the recipient strain and GMI1000 correlated with greater decreases in transformation frequencies (Figure 5).
In contrast to the single copy of comE1 in the Pasteurellaceae, there are four copies of comE in the N. gonorrhoeae genome and serial deletions resulted in decreases in transformation frequencies, with a reduction of 4×104-fold 4×104-foldwhen all copies wereductiond [6].
Formation of the γ phase influenced the composition of the bcc/B2 matrix, leading to decrease in martensitic transformation temperatures and transformation entropy change.
This result was attributed to the significant increase in the strength of the martensite phase and the large decrease in martensitic transformation finish temperature (Mf) by microstructural refinement.
With increasing Ti content, the amount of the Laves phase increases, which results in an increase in the thermal hysteresis and a simultaneous decrease in the transformation temperatures.
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