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Functional experiments revealed a dramatic decrease in the virulence of 05ZYH33 following the disruption of the TCS in the 89K GI [15].
In conclusion, we observed a remarkable decrease in the virulence of Nocardia brasiliensis HUJEG-1 after subculturing it 130 times in vitro, compared to the parent strain.
As such, the decrease in expression of this regulator under NaCl- and KCl-induced stress may be associated with a decrease in the virulence of the pathogen.
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To assess whether the inactivation of the oppD gene in C. pseudotuberculosis resulted in a decrease in the virulence profile of the mutant strain, infection assays using BALB/c mice were performed.
Reduced virulence of TC-adapted KFDV: Bhatt and Anderson have reported that the passage of the prototype P9605 strain of KFDV in monkey kidney tissue culture (MKTC) resulted in a decrease of the virulence of the virus for adult mice as judged by a decrease in LD50 titer of the virus following intraperitoneal (IP) inoculation.
In animal model, pretreatment of phytoestrogen-rich, Pueraria mirifica tuberous powder resulted in decreasing the virulence of rat breast tumor development induced by 7,12-dimethylbenz(a)anthracene (Cherdshewasart et al., 2007).
In addition, the mutation decreased the virulence of the bacteria in macrophages in vitro and in a mice model in vivo.
Our findings support the hypothesis that the use of ketorolac in the perioperative period of cataract surgery may decrease the virulence of S. epidermidis, the most common microorganism involved in post-cataract endophthalmitis.
Published evidence indicates that mutations in one or more of these putative virulence genes are sufficient to decrease the virulence of S. suis.
Particularly, it has been shown that deletion of evpP in E. tarda significantly decreased the virulence of the pathogens in fish [ 29].
Impairing the secretion of EvpC will decrease the virulence of the pathogenic bacteria.
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