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Results showed that cyclopamine alone to tumorsphere cells could make a small but consistent decrease in the viable cell number.
Also, an incubation with 100µM THPTS followed by illumination, yielded a 6lg (≥99.999%) decrease in the viable numbers of all bacteria strains tested, indicating that the THPTS drug has a high degree of photodynamic inactivation.
A bacteriostatic effect was defined as any decrease in the viable count from the starting inoculum [ 17].
In contrast, SCC4 and BaP-T cells underwent rapid arrest of replication upon infection with LV-Bmi-1i and showed drastic decrease in the viable cell number.
Bactericidal activity was defined as a ≧3log10 CFU/mL decrease in the viable cell counts within 24 hours with respect to the original inoculum.
Synergistic effect was defined as a ≧2log10 CFU/mL decrease in the viable cell counts compared to the most active drug.
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Kruskal-Wallis tests showed significant decreases in the viable cell count at 2, 4, 6 and 24 h after the addition of colistin compared with aztreonam alone or the 3-drug combination of aztreonam, ceftazidime and amikacin.
For trypsin incubation times greater than 10 min, especially over multiple ESC passages, a more dramatic decrease in the final viable cell number (∼80%) and Oct-4-GFP expression (∼70%) was measured.
For non-stimulated primary blood lymphocytes (PBLs) exposed to 1 10 LD (made from 3 ml/30 ml stock) there was a decrease in the number of viable cells in G0 as time passed, however viable cells were still present after 72 hours of exposure (Figures 9A, B and 9C)).
The time dependent killing of CA04 by the compounds revealed a decrease of >1log10 in the viable count compared to the control at 8 h by cinnamaldehyde and eugenol.
After 24 h of TTHL (IC80) treatment, approximately 55% MCF-7 cells were in early apoptosis (labeled with annexin V-PE), 15% cells were in late apoptosis (labeled with both annexin V-PE and 7-AAD), and a remarkable decrease was observed in the viable cells population (unlabeled with either annexin-V or 7-AAD) compared with the untreated control cells.
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