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While non-induced control cells were motile and locomoted over the course of the time-lapse, there was a significant decrease in the locomotion of TbHydin RNAi-induced cells with the majority of TbHydin RNAi-induced cells completely failing to locomote.
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In the experiment, the extract showed a noticeable decrease in locomotion in the test animals from the second observation period to last study period at both dose levels.
In Open field test, both LM100 and LM200 showed a noticeable decrease in locomotion in the test animals from the second observation period to the end of study period.
Ethanol causes a dose-dependent decrease in the rate of locomotion of C. elegans.
There is a clear correlation (Fig. 2B) between the decrease in locomotion and the arthritis score (Pearson r = -78, p = 0.0001).
Consistent with our previous findings in male Sprague-Dawley rats [ 22], the decrease in locomotion during the initial inhibitory phase was not due to an increase in rearing or grooming because these parameters were reduced during this interval.
No, or little, decrease in spontaneous locomotion in the open-field test has been reported previously for Shank3-deficient mice (Peça et al., 2011; Wang et al., 2011; Yang et al., 2012).
Although differences in open-field locomotion were found, the decrease in locomotion over consecutive test sessions contradicts that this behaviour is (exclusively) mediated by fearfulness in the rabbit.
Repeated exposure to another commonly used behavioural test, the elevated maze, has been reported to result in a decrease in total locomotion and a decrease in the amount of time spent on aversive open sections of the maze (e.g. Cook et al., 2002; Dawson et al., 1994; Rodgers et al., 1996).
The exacerbation of the decrease in locomotion over time is consistent with many studies showing that climbing ability decreases as a function of age [ 36- 38].
The decrease in locomotion activity by diazepam treated mice compare with the control may be due to the dose (400 mg/kg) used in the test that can produce sedation in mice [ 52].
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