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These results demonstrate a dose-dependent decrease in population cell doubling after exposure to IR (Fig. 1B).
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In both cell lines, following release from serum starvation, an increase of cell population in S phase and a decrease in cell population in G1 phase (data not shown) were observed, indicating that the cells entered a cell division cycle.
Conversely, inhibition of endogenous miR-383 with antisense oligonucleotides resulted in a significant elevation in cell proliferation, a decrease in cell population in the G1 phase and suppression of basal apoptosis.
The first phase of decrease in cell population is coincident with the increase of cell apoptosis induced by the treatment of docetaxel and paclitaxel, which suggest that taxane-induced apoptosis is likely responsible for the reduction of cell population at the doses less than 1 μM.
Similarly, NUF2 knockdown in Sw1990 cells also resulted in a significant increase of cell percentage in the G0/G1 phase and a concomitant decrease in cell population of G2/M phase.
The decrease in cell population during meniscal degeneration is not only related to the limited regenerative ability but also to accidental cell death (necrosis) and programmed cell death (apoptosis).
Mithramycin treatments demonstrated similar trends and showed a clear decrease in cell population between single agent and combination treatments.
Treatment with higher ratios of fish oil led to G1 arrest and a significant decrease in cell population in G2 M and S phase with reduced expression of cyclin D1 [ 18, 19].
BisGMA evoked an increase in the S cell population accompanied by a decrease in G2/M cell population (Fig. 8).
A decrease in meniscal cell population complicates the maintenance of a normal meniscus extracellular matrix and, therefore, alters the normal meniscal functions.
Therefore, a decrease in the cell population was observed at the beginning of the seventh cycle up to 2.6 g/l.
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