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c-FOS overexpression endowed the SKOV3 cells with a higher invasive phenotype, whereas a strong decrease in invasion of c-FOShigh OVCAR8 cells compared with control cells was observed (Supplementary Figure S1).
For example in melanoma, SOX2 knockdown in A2058 cells resulted in a 4.5-fold decrease in invasion in vitro and adopted this phenotype via the upregulation of matrix metalloproteinase (MMP -3 [49].
Cells treated with vehicle showed no decrease in invasion (Fig. 7).
Cells treated with 30 ug/ml of Methylene Blue saw a 50% decrease in invasion (p<0.05).
When cells in our invasion assay were treated with 40 nM Batimastat, we observed a similar decrease in invasion as seen with the Hsp70 or Hsp90α inhibitors.
Similarly, we observed a significant decrease in invasion of HepG2 cells transfected with C2GnT1 shRNA compared to cells transfected with a control vector, (mean number of cells/field = 14 versus 99, p<0.0005) (Fig. 6B, E, and F).
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For BT549 sh742 and sh528 - transduced tumor cells, 60%and40%0% decreases in invasion through Matrigel™ were observed.
To evaluate the effect of DIM on invasion of thyroid cells, all the cell lines were treated with 25 µM DIM (black bars) and a significant decrease (p<0.05) in invasion was observed.
However, the Gamba 05 strain (complement-resistant strain) maintained similar invasion rate in the presence of serum (Figure 4B), indicating that the decrease in cell invasion was caused by complement-mediated lysis.
A two-hour pre-incubation of IPEC-J2 cells with EcN resulted in a decrease in Salmonella invasion efficiency, while pre-incubation with E. coli 140815 or E. coli MG1655 did not.
In addition, knockdown of the T-UC.300A transcript resulted in a decrease in cell invasion (p=0.008).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com