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Furthermore, it is observed that a slight decrease in foci intensity appears on the field profile with a more random nanohole array.
A subset of these samples (n=CL-5; RS=13) were analysed at 24 h post-IR and showed a further decrease in foci number, approximately to background levels.
However, after 48 h post radiation we clearly observed a decrease in foci for pmiR-210 A549 cells compared with pmiR-Ctl cells (5.6±1.2 versus 10.8±3.3; Figure 5c).
Six of 10 patients show a marked increase in γH2AX foci formation following a PET scan exposure, 1 patient had a marked decrease in foci formation after the scan, and 2 patients displayed no change.
During the 24-h monitoring, the decrease in foci number was significantly slower in XRCC4-mutant fibroblasts (XRCC4 m/m #1, from individual II-1; XRCC4 m/m #2, from individual II-2) compared to that in wt cells, whereas at the endpoint, the differences became statistically non-significant (Fig 2A).
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Interestingly, KAN-1 also showed a decrease in focus numbers, but at the same time, an enlargement in size.
Ten days after incubation in 10 μg/ml and 100 μg/ml P. indica root aqueous extracts, GBM8401 cells showed diminished focus formation, and HeLa cells showed marked decrease in focus formation after 100 μg/ml P. indica root aqueous extract treatment compared to untreated cells in both cell lines.
Furthermore, SPOP-wt increased RAD51 foci formation compared to controls, while SPOP-F133V-expressing cells showed a dramatic decrease in RAD51 foci formation, consistent with impairment of HDR.
Decrease in TRF1 foci signal was accompanied with a significant increase in γH2AX-positive cells in both intestines and lung tumors (Fig 8D).
Somatic centromere labeling through p35S-CENH3-GFP shows a maximum of ten centromeric dots in young dividing tissues, reflecting the diploid chromosome number (2x = 10), and reveals a progressive decrease in GFP foci frequency throughout plant development.
Indeed, while treatment with the ATM inhibitor led to a dramatic decrease in γH2AX foci, inhibition of DNA-PKcs did not result in any detectable impairment of the γH2AX signal.
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