Sentence examples for decrease in dUTPase activity from inspiring English sources

So a decrease in dUTPase activity increases the occurrence of recombination events in Arabidopsis.

To investigate whether the decrease in dUTPase activity resulted in increased breaks in the genomic DNA, as in Trypanosoma [15], we performed a TUNEL assay.

In E. coli, dut-1 is synthetically lethal with a recA mutation [11], suggesting that DSBs resulting from a decrease in dUTPase activity are repaired by HR.

To examine if this reduction in DUT1 expression correlated with a decrease in dUTPase activity in the RNAi/DUT1 lines, protein was extracted from the leaves of WT, RNAi/DUT1-4 and RNAi/DUT1-5 plands andUTPasese activity was measured at 30°C using the crude extracts.

In this article, we describe the consequences of a reduction in dUTPase activity in plants, in the continuity with our very preliminary published data [16].

However, one might expect that by extending the homology, somatic HR via a reduction in dUTPase activity might become very efficient.

The RNAi/DUT1 plants were effectively depleted in dUTPase activity, and showed sensitivity to 5FU.

Decrease of dUTPase activity in Trypanosoma (Trypanosoma brucei), also results in the introduction of DSBs, as chromosome fragmentation is observed [15].

Finally, our data show that HR is probably increased in the RNAi/DUT1 plants provided, and only when, the RNAi is efficient at decreasing the dUTPase activity (which is ascertained by the 5FU sensitivity of the transgenic RNAi/DUT1 plants).

This observation suggests that repair of DSBs resulting from a decreased dUTPase activity is not entirely dependent on HR or on AtRAD51 as suggested from its induction.

Since no insertional mutants were available in Arabidopsis, we obtained, via a RNAi strategy, plants in which dUTPase activity was partially decreased.