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At 32°C, however, both coxibs induced a clear decrease in cell number owing to massive apoptosis in daunomycin-treated cells.
However, the net decrease in cell number was greater than that predicted solely from mitotic arrest.
This decrease in cell number causes the heart to become weaker with age, and it also causes Parkinson's disease and impairs theimmune system.
An increase or decrease in cell number results in a concomitant change in the amount of formazan produced, indicating the degree of cytotoxicity caused by the test material.
Similarly, compounds causing a decrease in cell number wereconsidered as toxic chemicalsand disregarded.
Compounds causing a decrease in cell number to fewer than 4,000/well were disregarded as toxic chemicals.
The decrease in cell viability (Fig. 2A) was found to correlate with a decrease in cell number (Fig. 2B).
No decrease in cell number or viability was observed in any of the experimental conditions as indicated by MTT assay (Figure S1).
As shown in Fig. 4A, incubation of cells with the ACC inhibitor for 48 h led to a ∼30% decrease in cell number compared to vehicle-treated controls.
The smaller decrease in ATP content relative to the decrease in cell number measured via trypan blue exclusion is consistent with previous cytotoxicity studies [27].
However, we observed a slight decrease in cell number in Scd1-treated NHDF that a block or a slower rate of proliferation could explain.
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