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There was also a significant decrease in cell capacitance.
However, paired comparisons revealed a statistically significant decrease in cell capacitance after cholesterol depletion (Fig. 6D, n = 18, P < 0.0001).
Extraction of membrane cholesterol with MCD led to a significant increase in the whole cell outward current and a significant decrease in cell capacitance.
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Wang et al. [ 40] found a decrease in current density, but no change in cell capacitance, studying rat tail arteries.
In another series of experiments (see Fig. 2D), changes in cell capacitance were monitored in uninfected cells (i.e. cells not expressing P2X2Rs) to allow the time course of ATP release to be compared with the increase in membrane capacitance.
The concurrent increase in cell capacitance, as with chronic MI in rats, infers cell size increase; confirmed here and in studies of chronic VTP or AF.
The fusion of insulin granules with the cell membrane can be measured as an increase in cell capacitance in response to a train of depolarisations [ 42].
Control superfusion of cells with physiological saline solution instead of MCD for 10 min produced no change in cell capacitance (Fig. 6D).
We also did not detect any difference in the cell capacitance or membrane resistance of LA principal cells according to age or genotype (Table S1).
In terms of biophysical membrane properties, the present findings demonstrated a significant sub-regional gradient from DLS to NAcC and NAcS, whereby the cell capacitance decreased and the Rin increased, consistent with morphological findings that somatic size and the number of primary/secondary dendrites of MSSNs decreases from DLS to VMS.
Cell A shows excellent long-term cyclic stability (less than a 5% decrease in specific capacitance after 1000 cycles).
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