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B Reperfusion time-dependent decrease in aconitase activity.
It is likely that the decrease in aconitase activity under these experimental conditions also involves other types of protein oxidation such as carbonylation and nitrosylation (Han et al. 2005; Yan et al. 1997).
Cells exposed to tellurite exhibited an important decrease in aconitase activity (Table 2).
A similar decrease in aconitase activity was evident in both cell lines upon transfection of mimic-210 (Figure 2A).
We found that Magmas mutants had a significant decrease in aconitase activity.
As expected, assays of injured cornea demonstrated a rapid decrease in aconitase activity, a reflection of an increase in ROS (Fig 2K).
Similar(50)
While Q0-YFP and Q30-YFP producing yeast cells show very similar aconitase activities, we observed a strong reduction in aconitase activity in Q56-YFP yeasts (Fig. 6b).
IRPs are also regulated by oxygen tension (reviewed and discussed in Refs 3, 8), which differentially regulates the binding activity of the two IRPs, with a decrease of IRP1, accompanied by a rise in aconitase activity, and an increase of IRP2 at low oxygen concentrations.
These results indicate that there was a time-dependent loss in aconitase activity in this in vitro oxidative stress system.
Impairment of respiratory chain complex activities and age-dependent defects in aconitase activity were also observed, which is associated with irregular and dysmorphic mitochondria in the skeletal muscle.
Usually, this drop in aconitase activity is compensated by the increased production of aconitase A (AcnA), which is invulnerable to oxidative inactivation in vivo [ 39, 49].
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