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Differences between age groups (stratified by decades) were calculated using a one-way analysis of variance (ANOVA) and multiple comparisons using a Scheffé test.
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Then the warm season (May-September) mean temperature at each grid point and each decade was calculated using the infilled dataset for the comparison with the reconstructions.
Moreover, these figures were calculated using census and mortality data half a decade out of date.
Averages were calculated using three spike-ins.
Modeled scattering intensities were calculated using CRYSOL63.
Read counts were calculated using HTSeq-count.
Relative expression levels were calculated using 2−△△Ct method2−△△Ct
Component scores were calculated using regression models.
Cd31+, Ng2+ and isolectin+ areas were calculated using ImageJ software.
The relative expression levels were calculated using the 2−ΔΔCt method2−ΔΔCt
Colony size and RFI of colonies were calculated using ImageJ.
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