Sentence examples for debris fractions from inspiring English sources

The worms were homogenized and separated by low-speed centrifugation (845 g, 5 min desktop centrifuge) into soluble [ post debris supernatant (PDS)] and insoluble (debris) fractions.

To solubilise and inactivate virus, the PEG precipitated concentrates of virus and the cell bound virus of the cell debris fractions were treated by adding Triton x-100 (Merck) to a final concentration of 0.1%, 0.5 and 1% (v/v).

Flow cytometry analysis revealed that compound 4i showed drastic cell cycle perturbations due to concentration dependant increase in the sub-G0 region which comprises of both the apoptotic and debris fraction, thus implying the extent of cell death.

Nuclear and cell debris fraction was isolated by centrifugation at 800 g for 10 min at 4°C.

HCT15 cells exposed to 15 and 30 µM of Plumbagin exhibited continuous increase in sub-G1 fraction which may include both apoptotic and debris fraction implying together the extent of cell death.

Both fractions were treated with 1.0% Triton x-100, in addition the cell debris fraction was centrifuged to remove residual cell debris, before the evaluation.

The virus content of the cell debris fraction and the PEG concentrated virus was preliminary evaluated in an ELISA by serially dilutions.

Three variants of virus and control antigens were tested; the antigen concentrated by PEG saturation (A), antigen from the cell debris fraction (B) and a combination of PEG precipitatedted antigen and cell debris antigen (A + B).

Seeds were harvested and crushed in liquid nitrogen then extracted consecutively with hexanes (lipid fraction), 0.5N HCl (phytate-containing acid-soluble fraction) and trifluoroacetic acid (TFA-soluble cell debris fraction).

Both the pelleted cell debris fraction and the PEG concentrated virus were then treated with Triton x-100 at three different concentrations of 0.1%, 0.5%, 1.0%, to explore the concentration required for an optimal solubilization and virus inactivation.

Worm debris (insoluble fractions) were supplemented with 120 μL PBS, 40 μL LDS sample buffer and 10 μL, reducing agent (Invitrogen, Carlsbad, CA, USA) boiled for 10 min and separated on 4 12% Bis Tris gels (Invitrogen; cat #NP0322).