Since protein lysates are highly complex with contaminants such as cellular or tissue debris, fats, hydrophobic protein aggregates, nucleic acids, and proteases that can directly and negatively affect the results from western blots, it is important to use cell lysis buffers and homogenization techniques that eliminate their effects [ 17].
The samples were then centrifuged at 17,000 xg for 30 min to remove the excess cell debris and fat.
Partially digested tissue was then homogenised using a Dounce homogeniser and centrifuged at 490 g in Sorvall SS-34 rotor to remove cell debris and fat.
Ethyl acetate is used as an extractor of debris and fat from the feces and leaves the parasites at the bottom of the suspension.
Tissue extracts were then separated from debris and fat by centrifuging at 13,000 rpm for 15 min. Protein content was measured by Bradford assay and 200 μg tissue protein was subjected to electrophoresis on a 10% SDS polyacrylamide gel followed by transfer onto nitrocellulose membranes.
Raw chicken feathers are wastes that are mixed with offal fat, debris, blood, preen oil and other wastes from the poultry process.
After harvesting, the grafts were cleaned and rinsed thoroughly, to remove bone debris and marrow fat.
For measurement of COX-2 and PPAR γ levels, the tissue specimens (100 mg) were minced, sonicated in 10 m M Tris-HCl, pH 7.4, 0.5 M NaCl and centrifuged at 10 000 g for 15 min at 4°C to separate cell debris and the fat layer.
In his introductory remarks, Nick Petrakis (UCSF School of Medicine, San Francisco, CA, USA) referred to breast fluids as, "a 'stew' containing exfoliated epithelial cells, foam cells, lymphocytes, disintegrating cellular debris, milk proteins, fat globules, fatty acids, enzymes, immunoglobulins, and other chemical substances derived from endogenous and exogenous sources".
