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Demographic characteristics, severity scores, injury type, aetiology of high fall, mortality rate and aetiology of death were analysed.
Morphology, functionality and cell death were analysed at different time of the culture.
Time to recurrence and death were analysed using a Kaplan Meier analysis with Log rank test.
The causes of death were analysed by forensic autopsy protocols with toxicological analyses (AN).
The data for cell survival and death were analysed using unpaired two-tailed t-test.
Time to first stroke and time to death were analysed using Cox proportional hazards regression.
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Further the mechanism of cell death was analysed by fluorescent microscopic analysis and also by scanning electron microscopy.
Cell death was analysed by counting the number of colony-forming units (CFUs), and compared with the untreated cell crude extract.
Cell death was analysed through Cell Death Detection ELISA (Roche).
Cell death was analysed 24 hours after simvastatin treatment.
The number of Brdu+ cells in each cerebellar layer at the different survival time has been analysed with Student's test T. A value of p≤0.05 was accepted as statistically significant The amount of apoptotic cell death was analysed by counting TUNEL-labelled cells in the cerebellum of 3, 4 and 36 months old animals (n = 3).
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