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Cell death was monitored by measuring guanine concentration in cells using differential pulse voltammetry (DPV), a simple and rapid electrochemical method, and was further confirmed by directly observing cell morphologies cultured on the transparent indium tin oxide (ITO) glass electrode and checking their viabilities using a trypan blue assay.
Development of cell death was monitored over time.
Percent worm death was monitored by counting the number of dead worms per 50 total worms found in a contiguous area under a dissecting scope.
Apoptotic cell death was monitored in the late third instar larval brains by immunohistochemical analysis of activated CASPASE-3, as described above.
F. tularensis subsp. novicida induced cell death was monitored using the Lactate Dehydrogenase-based In-Vitro Toxicology Assay kit (Sigma, Saint Louis, Missouri, USA).
As seen in Figure S1A, the viral protein VP2 could be detected in IPNV-infected ZF4 cells at 6, 9, and 12 h p.i. Next, ZF4 cells were infected with different viral doses, and cell death was monitored using a viability assay (Figure S1B).
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Signs of disease or death were monitored on a daily basis up to 30 days after infection.
After App infection, clinical signs of the disease (increased respiration rate, dyspnea, cough, anorexia, lethargy, death) were monitored and recorded twice a day.
Further, in lymphoma cells derived from Eμ-Myc/Raidd− / − mice, cell cycle distribution, spontaneous and drug-induced cell death were monitored and found comparable to their wt counterparts.
Signalling through apoptotic pathways and extent of cell death were monitored by western blot analysis and chromatin condensation by Hoechst staining, respectively.
Mortality rates and causes of death were monitored every year until January 2009 by systematic review of hospital records and death certificates from the Death Registry Office of Catalonia, coded according to the International Classification of Diseases (ICD) 9th edition.
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