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Their absorbance was recorded on a microplate spectrometer at 570 nm, and the percentage of cell death was calculated relative to untreated cells.
Specific death was calculated with the following formula: 100-[ x-background signal)/(untreated-background signal)x100-[ x-background
The mean age at death was calculated for each time period cohort of individuals.
The percentage cell death was calculated individually for each dose as follows: (ODcontrol−ODtreated/ODcontrol)×100, as described earlier [49].
The percentage of cell death was calculated by counting the number of EGFP-positive cells with condensed chromatin over the total number of EGFP-positive cells using a Nikon Eclipse TE2000-U microscope.
Time to death was calculated from the ICU admission date.
Shrimp mortality of each group was recorded, and the average time to death was calculated accordingly.
Hence, the risk of death was calculated to be 4.0 per 100 child-years of observation.
Risk ratio of death was calculated using the Cox regression risk model.
Overall survival from GK to death was calculated by the Kaplan Meier method.
The incidence rate (IR) of death was calculated as cases per person-year.
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