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Therefore, logarithmically growing cells were incubated with increasing concentrations of the hexane extract of M. philippensis (1 and 1.5 mg dry roots equivalent/ml medium) for 48 h and the induction of cell death was analyzed.
The following day, cell death was analyzed by staining with AnnexinV-Cy-5 following the manufacturer's instructions (BD Biosciences).
Cell death was analyzed by quantifying extracellular lactate dehydrogenase (LDH) activity with the cytotoxicity detection kit of LDH (Roche Applied Science), as described [68].
Cell death was analyzed ex vivo, at the time of sacrifice and after 24 h of culture (in vitro) by annexin V and 7AAD staining and flow cytometry analysis.
Since it is known that nicorandil can act as a vasodilator by activating sarcolemmal and the mitoK ATP) channel and by donating nitric oxide [15], the mechanism of the inhibitory effect of nicorandil on R120G induced cell death was analyzed in the cardiomyocytes.
Cell death was analyzed by flow cytometry.
Cell death was analyzed as described above.
The proportion of cell death was analyzed by MTT assay.
Cell death was analyzed by nuclear Hoechst staining.
No preventable but one potential preventable death was analyzed.
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Cell viability/death was analyzed by quantification of ATP levels and microscopical evaluation of propidium iodide (PI) uptake.
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