These "death cells" are described as completely inhumane, with no windows, electric light or proper sanitation.
For exclusion of death cells, samples were stained with Hoechst 33342 (Invitrogen).
"The prison was getting overcrowded," he had said, and a captain "said he was going to have to start executing the prisoners, the ones in the death cells".
This chimeric protein selectively sensitizes to apoptotic death cells derived from Simian-virus-40-transformed mouse fibroblasts (SVT2 cells).
During apoptotic death, cells are neatly carved up by caspases and packaged into apoptotic bodies as a mechanism to avoid immune activation.
To prevent such stress-induced death, cells usually stimulate only one of the pathways if the other is blocked or attenuated by stress (see detailed discussion below).
As shown in Figure 7d, after the protoplasts were treated with SWCNTs for 48 h, the death cells reached 80% or so, appeared yellow leaves, compared with the control group, there exists statistical difference between two groups, P < 0.05.
For counts of cell death, cells were stained with 0.04% Trypan blue and counted in a hemocytometer.
To induce death, cells were exposed to exogenous oxidative stress using H2O2 at the dose and time points indicated.
Cells death: Cells viability was evaluated by immunohistochemistry using Annexin V Fluos, an antibody coupled to FITC (Figure 2).
Death cells were confirmed by propidium iodide (PI; BD Pharmingen, San Diego, CA) staining and were excluded before analysis.
