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Since all animals remained in the study until end-stage of disease, typically a result of cumulative motor neuron death, analysis of motor neuron death in our study is likely to be uninformative.
Cell death analysis of TCTP-overexpressing HeLa cells was performed following etoposide treatment to assess the mitochondria-dependent apoptosis.
Mice were killed at 10 12 weeks (−15 weeks for cell death analysis) of age by cervical dislocation as approved by the UK Home Office Animal Scientific Procedures Act, 1986 (PPL 70/7349), with ethics approvals.
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The use of multiple cause of death analysis is one of the principal characteristics and advantages of the present study.
Cause of death analysis, the disaggregation of mortality and aging processes into specific ailments has been integral to demographic and gerontological studies of aging (Elo and Drevenstedt 2004; Finch 2009).
One of the principal resources derived from the use of multiple cause of death analysis is the recovery of data related to the associated causes of death listed in the medical certification section of the death certificate.
The objective of the present study was to employ multiple cause of death analysis in the study of trends in mortality related to dermatomyositis and polymyositis in the state of São Paulo between 1985 and 2007.
This methodology, designated multiple cause of death analysis, furnishes information regarding all of the conditions and circumstances involved in the process that culminated in death, offering new elements and perspectives for prevention [ 14, 15].
The mean number of causes of death listed per death certificate constitutes one of the principal indicators regarding multiple cause of death analysis [ 14, 15, 18].
For cell death analysis, 3.5 × 103 cells/cm2 of the indicated genotype were co-transfected with SureSilencing® shRNA and pIRES2-eGFP plasmids (3:1 ratio).
A cause of death analysis was performed.
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