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Only the live viable spores, separated from dead spores under a stereo microscope, were used for DNA extraction.
Equal volumes of dead spores were mixed with live spores of indicated strains at the same concentration.
This again suggests that excessive immune responses induced by dead spores of color mutants may enhance the larva killing effect of wild type A. fumigatus strains.
By inferring the genotype of the dead spore using the 1st Law of Mendel (which defines segregation), they noted that the majority of dead spores would have contained a recombinant chromosome III.
All infected larvae died within three days post inoculation, except the control group infected with wild type B5233 dead spores mixed with wild type B5233 live spores (Figure 10B).
If over-reactive immune responses can be induced by dead color mutants, we speculate that the larvae will show increased mortality rates when infected with live spores of wild type strains with dead spores of color mutants.
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To analyse three viable spore tetrads, the genotype of the dead spore was predicated by analysing the genotypes of the viable spores assuming Mendelian segregation.
They based this proposal on the observation that recombination was six-fold higher in tetrads with only three viable spores than in the four viable spore tetrads and that the dead spore was preferentially recombinant.
It was also possible that the bacteria had been picked up naturally, from a sick cow, even one long dead; anthrax spores could lie dormant in the ground for decades.
The company wouldn't say why, but analysts noted that EA is launching a slew of new franchises–"Spore," "Dead Space," "Mirror's Edge" and others and they speculated that the company might not have a good handle on whether it can release the new titles on time or if they will sell well.
We have a powerful product slate for 2008 and beyond, with exciting releases planned for many of EA's well-established franchises, as well as important new franchises we are launching, such as "SPORE," "Dead Space," "Dragon Age" and "Mirror's Edge".
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