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Dead mites on the traps from A. dorsata, Thai commercial, and Primorsky colonies were retrieved by the use of a fine paint brush and examined for their injuries using a stereomicroscope at 40 × magnification.
The number of the dead mites was recorded 24 h after treatment.
In addition, no dead mites were observed after application with LB broth.
The core of the balls was essentially composed of dead mites, as is shown by the percentage of dead mites in each part of the ball (2% of dead mites ± 2.1 for the outer ball and 68% of dead mites ± 20.8 for the inner ball) and was significantly different between the two parts (t-test comparing the proportion of dead mites per layers, t = 12.01, df = 14, p<0.0001, N = 15).
The dissection of the balls also showed that more dead mites are found in the inner part of the balls.
Therefore, each ball is composed of one inner part composed of a higher number of dead mites (and some eggs) than in the outer layer.
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In Herd B, one dead mite was isolated from one sow 8 months following treatment (Table 1). 1 Parts of a dead mite.
From one sow in Herd A, parts of a dead mite were found 16 months following treatment.
As one dead mite was found in the skin scrapings from cat 3B, the amitraz bathing was continued for an additional three weeks.
Each dead mite was associated with a feeding tube that had undergone melanotic encapsulation similar to that seen by the authors in a related damselfly, Lestes forcipatus [ 15, 44, 45].
The demonstration of dead mange mites in ear scrapings following day 0 was not surprising, even though mites were absent in experimental trials within 28 days following efficacious treatment [ 6, 7].
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