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(b) Dead cell ratio changes with time.
X indicates a dead cell or a decayed virion.
Dead cell exclusion was done by DAPI staining.
Dead cells: a dead cell with exactly three live neighbours becomes alive in all other cases a dead cell will stay dead.
Dr. Horvitz also identified a number of genes that direct how a dead cell is eliminated.
Following, a quantitative single-step dead cell indicator, SYTOX Green Dead Cell Stain (Invitrogen; S34860), was used to identify the dead population using flow cytometry.
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Dead cells were detected with eFluor 780 live dead cell marker (2000X).
The Live-dead cell staining also suggested impaired cell viability in the siP27-MLN4924 group.
"We're in a dead-cell area," Janice says.
DNGR-1 senses necrosis and regulates CD8+ T-cell cross-priming to dead-cell-associated antigens.
No cytotoxicity was observed by the live/dead cell viability assay.
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