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(E – G ) Differences in the lag times before the onset of response deactivation (Lag Deactivation).
10.7554/eLife.02053.005 Figure 3. Changing the ratio of Gβγ to an effector affects response deactivation lag in a reconstituted system.
In agreement with the electrophysiological recordings of GIRK channel activity, BRET experiments showed that the increase in Gβγ stoichiometry over an effector results in prolongation of the response deactivation lag.
The results of these experiments are entirely consistent with electrophysiological recordings of GIRK channel kinetics in the native system and show increase in response deactivation lag time upon an increase in Gβγ stoichiometry over an effector.
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(D ) Quantification of a lag deactivation time as a function of Gβγ/GRK ratio.
The lag deactivation time (dotted line for the trace in black) is defined as the time that it takes to quench the BRET signal by 10% from its steady state value in the presence of an agonist.
Under these conditions, there are virtually no genotype-dependent differences in the lag time of GIRK deactivation upon agonist removal.
Time constants of activation (τact) and deactivation (τdeact) of HCN4 currents were determined by fitting currents with a mono-exponential function after the initial lag.
The lag has been described before for the wild-type neurons and noted to be present in both activation and deactivation phases (Otis 1993, JPhysiol; Sodickson 2006, J Neurosci Zhangg 2002, J Physiol).
"Lag!" "Lag lag lag".
Nothing is deleted by deactivation.
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