Exact(3)
The samples were diluted with nine volumes of HKG buffer (20 mM NaCl, 100 mM KCl, 1 mM CaCl2, 1 mM MgCl2, 10 mM pyruvate, 20 mM HEPES, pH 7.4) [17], and labeled with X-rhod-1 (2 µM) and DCF (1 µM) at 37°C for 30 min. Measurement with loaded mitochondria were performed in PBS0Ca buffer.
HM cells grown in 100 mm dishes were labeled with fura-2 (1 µM) alone to measure [Ca2+]i or together with DHE (1 µM), DAF-2 (0.5 nM), or DCF (1 µM) as needed at 37°C for 30 min in RPMI 1640 medium to measure [Ca2+]i and superoxide, NO, or peroxynitrite simultaneously.
Leaves were infiltrated with the dye solution (SOSG 5 μM, DCF 1 mM and proxF 1 mM) and illuminated with strong red light (λ>600 nm, 1600 μmol m-2 s-1) at 10°C.
Similar(57)
Take the IEEE 802.11 DCF [25] as an example.
DCFH reacts with hydrogen peroxide as well as hydroxyl radicals to become fluorescent DCF [56].
The IEEE 802.11 DCF [8] is based on carrier sense multiple access with collision avoidance (CSMA/CA).
In terms of wireless media contention, the legacy 802.11 [2] was originally based on distributed coordination function (DCF) [3] to support collision handling among wireless stations.
We investigated if SWCNTs causes ROS formation in the Arabidopsis mesophyll cells by using DCF(2',7'-dichlorofluorescin diacetate) fluorescence as a reporter of intracellular oxidant production.
The MAC protocol in this paper is IEEE 802.11 DCF [18], where carrier sense multiple access with collision avoidance (CSMA/CA) is adopted.
The IEEE 802.11p uses the enhanced distributed channel access (EDCA) medium access control (MAC) sublayer protocol based on distributed coordination function (DCF) [4].
Bianchi proposes a classic two-dimensional Markov chain to determine the saturation throughput of a Wireless Local Area Network (WLAN) using the Distributed Coordination Function (DCF) [15].
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