Six EDL and soleus muscles from age-matched (35±1 days) wild-type (n = 3) and lmna-null (n = 3) mice were fixed in 2.5% phosphate buffered glutaraldehyde (pH 7.3, 0.1 M) for 4 hours at 4°C.
We found mice that are deficient in Mmp13 to have a mean healing time indistinguishable from wild-type mice (16.3 versus 15.7 days), while Plau-deficient mice had a mean healing time that was delayed to 20.1 days (wild-type versus Plau−/−; p = 0.002) (Figure 2, A and B).
As above, we found mice that are deficient in Mmp13 to have a mean healing time indistinguishable from wild-type mice (17.0 versus 15.6 days), while Plg-deficient mice had a mean healing time that was delayed to 34.1 days (wild-type versus Plg−/−; p<0.001) (Figure 2, C and D).
When plants were grown under 10/14 h photoperiod for 48 days, wild-type produced approximately 30 rosette leaves, whereas rack1a-1 single mutant produced 22 rosette leaves.
After 2 days, wild-type cotyledons were reflexed, fully green and almost completely expanded, and the pale fln cotyledons were evident.
After 2 days, wild-type organs had undergone marked growth (Fig. 1G) whereas, in comparison, the progress of Vangl2 Lp/Lp organs appeared retarded to variable degrees (Fig. 1H and I).
After prolonged incubation on Ca2+-depleted solid minimal medium (>14 days), wild-type C. albicans colonies produced aberrant lobed margins that could be alleviated by the addition of 10 mM Ca2+ to the medium.
Measurement of the distance between the DiI-loaded beads and the most distal tangentially orientated DiI-labelled cells revealed that within 3 days wild-type cells had migrated a longer distance than their Gdf15 -/- counterparts (Fig. 7B).
Normally, mice entrained to a 12 12 LD cycle became active around the transition to lights-off, and after a 10 hr delay of this photoschedule, their activity-rest cycle achieved stable re-entrainment after 4.75 ± 0.19 days (wild-types) or 4.11 ± 0.39 days (heterozygotes).
