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Time-release pellets designed to deliver one of three doses of LPS: Low (3.3 μg/day), High (33.3 μg/day), or Placebo over 90 days, were implanted subcutaneously in 3-month-old male Sprague Dawley rats (n = 8/group).
Male Lewis rats fed with CDDO-MA-containing or control diet for 7 days were implanted subcutaneously with osmotic minipumps (Model 2ML1, Alzet, Cupertino CA, filled with 3-NP 54 mg/ml in PBS, pH 7.4) that delivered 3-NP 50 mg per kg bodyweight daily for 7 days while remaining on the same diets [46].
After 20 days of tumour growth, TMX releasing pellets (7.5 mg/60 days) were implanted subcutaneously, thus mimicking clinical scenarios in which a tumour mass has already grown prior to initiation of treatment.
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He thinks energy harvesters could be used to power robotic artificial joints and might even, one day, be implanted within muscles for that purpose.A green lightEven the quotidian uses of an energy harvester like Dr Donelan's will, however, be enhanced by other advances in the field.
Some scientists believe a cloned mammoth embryo could one day be implanted in an Asian elephant's womb.
After acclimation (8 to 27 days), cells were implanted on day 0. Each mouse was anesthetized with a mixture of isofluorane and oxygen, and about 5 × 106 cells, suspended in 100 μl cold (0°C to 4°C) serum-free medium, were injected into the right thoracic mammary fat pad through a 5-mm incision at the sternum region, by using a 1.0-ml syringe with 26-gauge needle.
Osmotic minipumps (Alzet mini-osmotic pump, model 2007, Durect Corp., Cupertino, CA) that maintain a constant flow over 3 or 7 days at 0.5 μL/h were implanted 20 days post tumor induction.
Two days later, they were implanted with morphine or placebo pellets (to induce dependence in morphine-pelleted animals) for 2 weeks, followed by forced abstinence.
Cultures of HUMSCs were treated with neuronal conditioned medium (NCM) for 0, 3 or 6 days, and then were implanted to the lesion site as well as the rostral and caudal stumps of the transected spinal cords.
After a recovery period of 1 2 days, tumor spheroids were implanted.
Osmotic minipumps (Alzet Model 1002, Charles River UK Ltd, Margate, UK) releasing either AngII at a dose of 1.1 mg kg−1 day−1 for 14 days or saline were implanted subcutaneously under isoflurane anaesthesia (2% isoflurane+98% O2).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com