Early (<100 days) and late mortality (≥100 days) was not differentiated in the studies.
3T3-L1 cells were cultured to confluence (day 0) and 2 days later, were differentiated as described for HIB-1B cells.
After 2 days, cells were differentiated with retinoic acid (10 μM) for 6 days prior to treatment.
Cells were treated for 3 days to be differentiated into neuronal-like cells [ 16].
3T3-L1 cells at 2 days post-confluence were differentiated into adipocytes using differentiation-inducing medium (1 µM dexamethasone (DEX), 0.5 mM methylisobutylxanthine, and 1.67 µM insulin in DMEM with 10% FBS).
Two days after plating, cells were differentiated using "Differentiation Medium" (catalog # DM-2, ZenBio) and considered as Day '0'.
After 2 days, ∼40×106 cells were differentiated with PMA as described above, infected with M. tb H37Rv for 3 4 h at a multiplicity of infection of 10∶1 per flask; washed with incomplete RPMI 1640 medium and incubated in fresh complete RPMI 1640 medium.
Neurospheres at 6 days after passage were differentiated as previously described.
Two days later, NSPCs were differentiated by changing the media to NBA containing 1X PSQ, 1X B27, and 1% FBS.
Human MSCs (cultured under micromass conditions for 21 days in hypoxia) were differentiated with conditioned medium derived from porcine notochordal cells in native tissue (NCT) or in alginate beads (NCA), and compared with chondrogenic (TGFβ-3) or basal medium.
