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The widely used 3T3-L1 mouse pre-adipocytes [18] differentiate within 6 days upon exposure to a very similar differentiation mix, although typically T3 is omitted and fetal bovine serum (FBS) is included in the medium.
In contrast, the nuclear/cytosolic ratio of β-catenin was much higher in Ad-Rspo1-treated mice in basal conditions (day –1, Fig 7B), which further increased by 2 4 folds the value of AdLacZ-treated animals, with a peak around 3.5 days upon exposure to WBI (Fig 7A and B).
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The repression of the SAR response that we observed 2 days upon NP exposure has been corroborated in experiments with Arabidopsis exposed for 10 days to Ag-NPs [ 18].
Figure 2c shows the proliferation rate (i.e. expansion factor over the course of 3 days) of MUTZ3 versus hTERT-MUTZ3 upon exposure to 30 nM doxorubicin.
Standard toxicological assays using the zebrafish model system evaluate lethality and teratogenicity upon exposure during the first 2 days after fertilization.
(49) The theophylline/citric acid cocrystal converted to a cocrystal hydrate upon exposure to 98% RH for 3 days.
It was found that the caffeine/citric acid cocrystal converted to caffeine hydrate upon exposure to 98% RH for 7 days, similar to the other caffeine cocrystals.
Upon exposure of the mutant to 50 μM As for 7 days in hydroponics, Cu/Zn SODs transcript level is reduced further, suggesting As-induced down-regulation of the gene.
The corrosion rate (PН) values for both types of the magnesium alloys (MA8 and VMD10) were about 0 mm per year upon exposure of the samples to the 3% NaCl solution for 7 days.
Upon exposure to high NaCl, these cells enlarge, flatten and become multinucleated within 2 days, and their cell cycle becomes permanently arrested [ 6].
A similar protective effect of lovastatin was found upon exposure of Balb/c mice to three consecutive doxorubicin doses and assaying subacute toxicity 8 days after the last doxorubicin treatment.
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