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From postnatal day 25, for 33 days, the animals were tested for behavioral changes using an operant procedure.
Mr. Ward said that within days, the animals had changed some wooded areas from impassable thickets to strollable glades.
After 14 days, the animals were sacrificed.
After 119 days, the animals were slaughtered.
During the last 7 days, the animals were moved to metabolism crates for total urine and fecal collection.
After a survival time of 7 8 days, the animals were perfused with a fixative and brain tissue was prepared for histological analysis.
After the experimental period (30 days), the animals were decapitated for the blood collection and testes were removed for the isolation of Sertoli cells.
After 360 days, the animals were killed by anesthetic overdose; then, the teeth were histologically prepared, sectioned, and stained with hematoxylin and eosin for optical microscopic analysis of apical and periapical tissue repair.
After a period of 35 days, the animals were sacrificed.
After 36 days, the animals were sacrificed and the tumors were confirmed by histological examination.
At the end of 14 days, the animals were sacrificed, weights of prostate and levator ani measured and the tissues collected for RNA isolation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com