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In the early days of tissue research, when chemical signaling was still far from being discovered, morphogenesis was understood as a purely mechanical phenomenon [ 491].
In an attempt to separate the effect of the external cue (food) from the potential internal mechanism, in the next set of experiments we assayed phosphorylation rhythms in animals that were maintained on the TR feeding regime for two weeks but did not receive food on the days of tissue collection; thus, mice were fasting for up to 52 hrs.
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On the day of tissue harvesting, bears were immobilized between 9 30 15:00 using Telazol (8 10 mg/kg) administered via pole syringe using a 16 g 1.5 inch needle or a CO2 powered pistol to administer Telazol via a standard Palmer dart.
On day 22 of pregnancy, the last day of tissue culture, the pregnant rats under ad lib and restricted feeding were fasted to prevent the direct effects of food intake on the fetal SCN, to make sure not just one feeding event, but repeated feeding cycles over day 0 21 of pregnancy contributed to any changes in the fetal SCN rhythms.
The treatment of fractures was delayed by 3 days because of tissue swelling.
In the early days of human tissue banking, not-for-profit banks, mostly located in hospitals, dominated the field.
(C ) Schema showing that LAP-MYC +/- let-7S21L mice were induced at 14 days of age, tissues were collected at 90 days of age, and survival was followed.
Rats survived for over 710 days after implantation of tissue constructs.
Tissue was obtained from patients at three time points: tissue from incision biopsy performed at presentation, tissue from tru-cut biopsy performed after 14 days of treatment and tissue obtained at definitive surgery.
To study whether MEK162 had sorted an effect on the leptomeningeal melanocytosis after 5 days of treatment, tumor tissue removed at operation before MEK162 treatment and tumor tissue at autopsy, was stained with the antibodies Ki67 (MIB-1) and pERK.
Samples were agitated at 150 rpm at 55°C for 4-6 days with the level of tissue digestion checked after 3 days: those which still had visible amounts of tissue had a further 10 μl of Proteinase K added for the remainder of the incubation.
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