Interestingly, miR156 expression was not affected during the first 9 days of induction, whereas that of EgSPL5 in mature cuttings decreased after 1 day and remained low.
The experimental design was achieved by four different stress media and two different light intensities for 14 days of induction period.
During the four days of induction of SARA, 0.30 of ad libitum intake of the total mixed ration (TMR) was replaced with wheat and barley pellets (WBP) containing 0.5 ground wheat and 0.5 ground barley.
Each period of the Latin square consisted of three weeks of adaptation to the respective treatments followed by four days of induction of SARA and three days of rest.
After 10 days of induction with intravenous ganciclovir, the patient was switched to oral valganciclovir 900 mg twice per day.
The growth starts with a thin amorphous layer at the interface of the two-phase mixture within 2 days of induction followed by slow growth of a white matrix of fibers attached to the thin amorphous layer in the water phase.
Proteoglycan deposition was checked by Safranin O staining after 15 days of induction [42].
After 15 days of induction, the 96-well test plates were read through the fluorescence spectrophotometer.
After 5 days of induction, the cells were fixed and stained for tartrate-resistant acid phosphatase (TRAP).
Undifferentiated cultures and cultures after 5 days of induction, containing at least 81% of differentiated cells, were processed.
After 21 days of induction toward an adipogenic lineage, a characteristic morphological change with accumulation of lipid vacuoles was observed (Figure 1B left).
