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For CAS agar assay, formation of orange siderophore halo was evaluated following 3 days of colony incubation at 37°C.
10) Figure 2A, how many days of colony growth?
After 14 days of colony growth, medium was removed and cells washed once in 1 ml of PBS.
After incubation in anaerobic jars at 55°C for 2 3 days, hundreds of colonies grew out.
After six days of growth, colonies were scanned, and the total area of the colonies from each sample was measured using the Image Pro Plus 5 software.
Plates were examined after 7 days of incubation: colonies were differentiated into macro-morphological types using dissection microscopy and the respective counts were recorded as colony forming units (CFU).
After 2 days of incubation, colonies appeared on the selective agar plate which showed resistance to the leucine analog.
After 7 days of growth colonies were detached mechanically from the feeder cells with a micropipette tip.
After 14 days of culture, colonies were counted using an inverted microscope (Axiovert-II Zeiss) and gridded scoring dishes.
After 14 days of incubation, colonies were counted and measured.
After 14 days of incubation, colonies were counted.
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