Tissues were sampled as follows: 1 cm crown sections after 30 days of cold treatment; 1 cm vernalized (49-day cold-treated) crown sections that were exposed to flower inducing conditions for 11 days; different developmental stages of spike formation (5 to 50 mm); and different developmental stages of spike and seed formation after the spikes had emerged from the flag leaf (visible).
Intriguingly, whereas 1 day of cold exposure did not affect Angptl4 expression, 10 days of cold exposure led to a marked reduction in Angptl4 mRNA.
After 42 days of growth, plants were transferred to a 4°C growth cabinet at 16 h day length with 90 μE m-2 s-1 for an additional 14 days of cold acclimation.
The other two people who died from the two days of cold were homeless.
After days of cold, even the most rugged New Yorkers might find themselves wishing for a warming trend.
Fewer people attended than organizers had said they hoped for, even though after days of cold, wet weather, the sun came out this morning.
The days of cold war pantomime journalism and great ideological battles might be over, but North Korea remains an area in which journalists have free licence for sensationalism and partiality.
Another 15 days of cold weather will turn Italy's gas situation into a "red alert," Eni's chief executive, Paolo Scaroni, told journalists from Il Messaggero, which published his comments yesterday.
The industry, four decades old, took root in the days of cold war military contracting and was fostered in the highly regulated, monopolistic world of government-owned telecommunications agencies and intergovernmental satellite organizations.
In the last few days of cold weather, which tends to coincide with Lent, there is a wonderful old tradition in Piedmont, cante le oeuf, which translates literally as "sing for the eggs".
Indeed, as the sun finally emerged here after several days of cold rain, and the city immersed itself in its weekday rhythms, fans didn't hide their anger about being left in the lurch.
