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Cells were also grown on Edinburgh minimal medium + supplements (ade-nine, histidine, lysine hydrochloride, and leucine) (EMMS-ura; ) at 30°C for 8 days for transformation with plasmids.
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Agrobacterium tumefaciens GV3101 strain containing a gene construct was cultured on the same day for transformation of these explants the next day.
I feared our days of transformation were behind us.
One hundred plants of a soybean cultivar (G. max cv. 'William 82') susceptible to parasitism by SCN and RKN were grown for each experiment for 9 days before transformation.
Plants were dipped for transformation after 1 week in long day and dipped a second time 6 days later.
Confocal microscopy two to four days after transformation demonstrated a plastidial localization of the SP-GFP fusions for SsLPPS and SsSS.
Using stem sections with one node as explants, a 2-day pre-culture of explants, infection with K599 at OD600 = 0.6, and co-cultivation on medium (pH 5.4) at 22°C for 2 days enhanced the transformation frequency significantly.
"I am for transformation," she answered.
Drag is about the quest for transformation.
Krepinevich calls these "poster children for transformation".
What are the possibilities for transformation?
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